Research Publications

2019

Nagy, P. D., and W. Lin. 2020. Taking over Cellular Energy-Metabolism for TBSV Replication: The High ATP Requirement of an RNA Virus within the Viral Replication Organelle. Viruses 12.

Wu, C. Y., and P. D. Nagy. 2019. Blocking tombusvirus replication through the antiviral functions of DDX17-like RH30 DEAD-box helicase. PLoS Pathog 15:e1007771.

Lin, W., Y. Liu, M. Molho, S. Zhang, L. Wang, L. Xie, and P. D. Nagy. 2019. Co-opting the fermentation pathway for tombusvirus replication: Compartmentalization of cellular metabolic pathways for rapid ATP generation. PLoS Pathog 15:e1008092.

Kovalev, N., J. Pogany, and P. D. Nagy. 2019. Interviral recombination between plant, insect and fungal RNA viruses: The role of intracellular Ca(++)/Mn(++) pump. J Virol.

Inaba, J. I., K. Xu, N. Kovalev, H. Ramanathan, C. R. Roy, B. D. Lindenbach, and P. D. Nagy. 2019. Screening Legionella effectors for antiviral effects reveals Rab1 GTPase as a proviral factor coopted for tombusvirus replication. Proc Natl Acad Sci U S A 116:21739-21747.

Feng, Z., K. Xu, N. Kovalev, and P. D. Nagy. 2019. Recruitment of Vps34 PI3K and enrichment of PI3P phosphoinositide in the viral replication compartment is crucial for replication of a positive-strand RNA virus. PLoS Pathog 15:e1007530.

2018

Sasvari, Z., N. Kovalev, P. A. Gonzalez, K. Xu, and P. D. Nagy. 2018. Assembly-hub function of ER-localized SNARE proteins in biogenesis of tombusvirus replication compartment. PLoS Pathog 14:e1007028.

Inaba, J. I., and P. D. Nagy. 2018. Tombusvirus RNA replication depends on the TOR pathway in yeast and plants. Virology 519:207-222.

2017

Xu, K., and P. D. Nagy. 2017. Sterol Binding by the Tombusviral Replication Proteins Is Essential for Replication in Yeast and Plants. J Virol 91.

Prasanth, K. R., C. Chuang, and P. D. Nagy. 2017. Co-opting ATP-generating glycolytic enzyme PGK1 phosphoglycerate kinase facilitates the assembly of viral replicase complexes. PLoS Pathog 13:e1006689.

Nagy, P. D. 2017. Exploitation of a surrogate host, Saccharomyces cerevisiae, to identify cellular targets and develop novel antiviral approaches. Curr Opin Virol 26:132-140.

Kovalev, N., J. I. Inaba, Z. Li, and P. D. Nagy. 2017. The role of co-opted ESCRT proteins and lipid factors in protection of tombusviral double-stranded RNA replication intermediate against reconstituted RNAi in yeast. PLoS Pathog 13:e1006520.

Fernandez de Castro, I., J. J. Fernandez, D. Barajas, P. D. Nagy, and C. Risco. 2017. Three-dimensional imaging of the intracellular assembly of a functional viral RNA replicase complex. J Cell Sci 130:260-268.

Chuang, C., K. R. Prasanth, and P. D. Nagy. 2017. The Glycolytic Pyruvate Kinase Is Recruited Directly into the Viral Replicase Complex to Generate ATP for RNA Synthesis. Cell Host Microbe 22:639-652 e7.

2016

Xu, K., and P. D. Nagy. 2016. Enrichment of Phosphatidylethanolamine in Viral Replication Compartments via Co-opting the Endosomal Rab5 Small GTPase by a Positive-Strand RNA Virus. PLoS Biol 14:e2000128.

Prasanth, K. R., N. Kovalev, I. F. de Castro Martin, J. Baker, and P. D. Nagy. 2016. Screening a yeast library of temperature-sensitive mutants reveals a role for actin in tombusvirus RNA recombination. Virology 489:233-242.

Nawaz-Ul-Rehman, M. S., K. R. Prasanth, K. Xu, Z. Sasvari, N. Kovalev, I. F. de Castro Martin, D. Barajas, C. Risco, and P. D. Nagy. 2016. Viral Replication Protein Inhibits Cellular Cofilin Actin Depolymerization Factor to Regulate the Actin Network and Promote Viral Replicase Assembly. PLoS Pathog 12:e1005440.

Nagy, P. D., J. R. Strating, and F. J. van Kuppeveld. 2016. Building Viral Replication Organelles: Close Encounters of the Membrane Types. PLoS Pathog 12:e1005912.

Nagy, P. D., J. Pogany, and K. Xu. 2016. Cell-Free and Cell-Based Approaches to Explore the Roles of Host Membranes and Lipids in the Formation of Viral Replication Compartment Induced by Tombusviruses. Viruses 8

Nagy, P. D. 2016. Tombusvirus-Host Interactions: Co-Opted Evolutionarily Conserved Host Factors Take Center Court. Annu Rev Virol 3:491-515.

Kovalev, N., I. F. de Castro Martin, J. Pogany, D. Barajas, K. Pathak, C. Risco, and P. D. Nagy. 2016. Role of Viral RNA and Co-opted Cellular ESCRT-I and ESCRT-III Factors in Formation of Tombusvirus Spherules Harboring the Tombusvirus Replicase. J Virol 90:3611-26.

2015

Xu, K., and P. D. Nagy. 2015. RNA virus replication depends on enrichment of phosphatidylethanolamine at replication sites in subcellular membranes. Proc Natl Acad Sci U S A112:E1782-E1791.

Tian, M., Z. Sasvari, P. A. Gonzalez, G. Friso, E. Rowland, X. M. Liu, K. J. van Wijk, P. D. Nagy, and D. F. Klessig. 2015. Salicylic Acid Inhibits the Replication of Tomato bushy stunt virus by Directly Targeting a Host Component in the Replication Complex. Mol Plant Microbe Interact 28:379-86.

Prasanth, K. R., D. Barajas, and P. D. Nagy. 2015. The Proteasomal Rpn11 Metalloprotease Suppresses Tombusvirus RNA Recombination and Promotes Viral Replication via Facilitating Assembly of the Viral Replicase Complex. J Virol 89:2750-63.

Pogany, J., and P. D. Nagy. 2015. Activation of Tomato Bushy Stunt Virus RNA-Dependent RNA Polymerase by Cellular Heat Shock Protein 70 Is Enhanced by Phospholipids In Vitro. J Virol 89:5714-23.

Nagy, P. D. 2015. Viral Sensing of the Subcellular Environment Regulates the Assembly of New Viral Replicase Complexes during the Course of Infection. J Virol 89:5196-5199.

Chuang, C., K. R. Prasanth, and P. D. Nagy. 2015. Coordinated Function of Cellular DEAD-Box Helicases in Suppression of Viral RNA Recombination and Maintenance of Viral Genome Integrity. PLoS Pathog 11:e1004680.

Barajas, D., N. Kovalev, J. Qin, and P. D. Nagy. 2015. Novel Mechanism of Regulation of Tomato Bushy Stunt Virus Replication by Cellular WW-Domain Proteins. J Virol 89:2064-79.

2014

Xu, K., and P. D. Nagy. 2014. Expanding use of multi-origin subcellular membranes by positive-strand RNA viruses during replication. Curr Opin Virol 9C:119-126.

Xu, K., J. Y. Lin, and P. D. Nagy. 2014. The hop-like stress-induced protein 1 cochaperone is a novel cell-intrinsic restriction factor for mitochondrial tombusvirus replication. J Virol 88:9361-78.

Sasvari, Z., P. Alatriste Gonzalez, and P. D. Nagy. 2014. Tombusvirus-yeast interactions identify conserved cell-intrinsic viral restriction factors. Front Plant Sci 5:383.

Nagy, P. D., J. Pogany, and J. Y. Lin. 2014. How yeast can be used as a genetic platform to explore virus-host interactions: from 'omics' to functional studies. Trends Microbiol 22:309-316.

Li, Z., P. A. Gonzalez, Z. Sasvari, T. G. Kinzy, and P. D. Nagy. 2014. Methylation of translation elongation factor 1A by the METTL10-like See1 methyltransferase facilitates tombusvirus replication in yeast and plants. Virology 448C:43-54.

Kovalev, N., J. Pogany, and P. D. Nagy. 2014. Template role of double-stranded RNA in tombusvirus replication. J Virol 88:5638-51.

Kovalev, N., and P. D. Nagy. 2014. The Expanding Functions of Cellular Helicases: The Tombusvirus RNA Replication Enhancer Co-opts the Plant eIF4AIII-Like AtRH2 and the DDX5-Like AtRH5 DEAD-Box RNA Helicases to Promote Viral Asymmetric RNA Replication. PLoS Pathog 10:e1004051.

Chuang, C., D. Barajas, J. Qin, and P. D. Nagy. 2014. Inactivation of the Host Lipin Gene Accelerates RNA Virus Replication through Viral Exploitation of the Expanded Endoplasmic Reticulum Membrane. PLoS Pathog 10:e1003944.

Barajas, D., K. Xu, M. Sharma, C. Y. Wu, and P. D. Nagy. 2014. Tombusviruses upregulate phospholipid biosynthesis via interaction between p33 replication protein and yeast lipid sensor proteins during virus replication in yeast. Virology 471-473C:72-80.

Barajas, D., K. Xu, I. F. de Castro Martin, Z. Sasvari, F. Brandizzi, C. Risco, and P. D. Nagy. 2014. Co-opted Oxysterol-Binding ORP and VAP Proteins Channel Sterols to RNA Virus Replication Sites via Membrane Contact Sites. PLoS Pathog 10:e1004388.

Barajas, D., I. F. Martin, J. Pogany, C. Risco, and P. D. Nagy. 2014. Noncanonical Role for the Host Vps4 AAA+ ATPase ESCRT Protein in the Formation of Tomato Bushy Stunt Virus Replicase. PLoS Pathog 10:e1004087. 

2013

Shah Nawaz-Ul-Rehman, M., K. Reddisiva Prasanth, J. Baker, and P. D. Nagy. 2013. Yeast screens for host factors in positive-strand RNA virus replication based on a library of temperature-sensitive mutants. Methods 59:207-16.

Sasvari, Z., N. Kovalev, and P. D. Nagy. 2013. The GEF1 Proton-Chloride Exchanger Affects Tombusvirus Replication via Regulation of Copper Metabolism in Yeast. J Virol 87:1800-10.

Sasvari, Z., P. A. Gonzalez, R. A. Rachubinski, and P. D. Nagy. 2013. Tombusvirus replication depends on Sec39p endoplasmic reticulum-associated transport protein. Virology 447:21-31.

Pathak, K. B., Z. Jiang, V. Ochanine, M. Sharma, J. Pogany, and P. D. Nagy. 2013. Characterization of dominant-negative and temperature-sensitive mutants of tombusvirus replication proteins affecting replicase assembly. Virology 437:48-61.

Lin, J. Y., and P. D. Nagy. 2013. Identification of Novel Host Factors via Conserved Domain Search: Cns1 Cochaperone Is a Novel Restriction Factor of Tombusvirus Replication in Yeast. J Virol 87:12600-10.

Kovalev, N., and P. D. Nagy. 2013. Cyclophilin a binds to the viral RNA and replication proteins, resulting in inhibition of tombusviral replicase assembly. J Virol 87:13330-42. 

2012

Xu, K., T. S. Huang, and P. D. Nagy. 2012. Authentic in vitro replication of two tombusviruses in isolated mitochondrial and endoplasmic reticulum membranes. J Virol 86:12779-94.

Shah Nawaz-Ul-Rehman, M., N. Martinez-Ochoa, H. Pascal, Z. Sasvari, C. Herbst, K. Xu, J. Baker, M. Sharma, A. Herbst, and P. D. Nagy. 2012. Proteome-wide overexpression of host proteins for identification of factors affecting tombusvirus RNA replication: an inhibitory role of protein kinase C. J Virol 86:9384-95.

Sasvari, Z., and P. D. Nagy. 2012. Identification of small molecule inhibitors of tomato bushy stunt virus replication. Methods Mol Biol 894:345-57.

Qin, J., D. Barajas, and P. D. Nagy. 2012. An inhibitory function of WW domain-containing host proteins in RNA virus replication. Virology 426:106-19.

Pogany, J., and P. D. Nagy. 2012. p33-Independent Activation of a Truncated p92 RNA-Dependent RNA Polymerase of Tomato Bushy Stunt Virus in Yeast Cell-Free Extract. J Virol 86:12025-38.

Pathak, K. B., J. Pogany, K. Xu, K. A. White, and P. D. Nagy. 2012. Defining the Roles of cis-Acting RNA Elements in Tombusvirus Replicase Assembly In Vitro. J Virol 86:156-71.

Nagy, P. D., and C. D. Richardson. 2012. Viral replication-in search of the perfect host. Curr Opin Virol.

Nagy, P. D., and J. Pogany. 2012. The dependence of viral RNA replication on co-opted host factors. Nature Reviews Microbiology 10:137-149.

Nagy, P. D., D. Barajas, and J. Pogany. 2012. Host factors with regulatory roles in tombusvirus replication. Curr Opin Virol 2:685-92.

Lin, J. Y., V. Mendu, J. Pogany, J. Qin, and P. D. Nagy. 2012. The TPR Domain in the Host Cyp40-like Cyclophilin Binds to the Viral Replication Protein and Inhibits the Assembly of the Tombusviral Replicase. PLoS Pathog 8:e1002491.

Kovalev, N., J. Pogany, and P. D. Nagy. 2012. A Co-Opted DEAD-Box RNA Helicase Enhances Tombusvirus Plus-Strand Synthesis. PLoS Pathog 8:e1002537.

Kovalev, N., D. Barajas, and P. D. Nagy. 2012. Similar roles for yeast Dbp2 and Arabidopsis RH20 DEAD-box RNA helicases to Ded1 helicase in tombusvirus plus-strand synthesis. Virology 432:470-84.

Barajas, D., and P. D. Nagy. 2012. Expression of dominant-negative mutants to study host factors affecting plant virus infections. Methods Mol Biol 894:359-76. 

2011

Stork, J., N. Kovalev, Z. Sasvari, and P. D. Nagy. 2011. RNA chaperone activity of the tombusviral p33 replication protein facilitates initiation of RNA synthesis by the viral RdRp in vitro. Virology 409:338-47.

Sharma, M., Z. Sasvari, and P. D. Nagy. 2011. Inhibition of phospholipid biosynthesis decreases the activity of the tombusvirus replicase and alters the subcellular localization of replication proteins. Virology 415:141-52.

Sasvari, Z., L. Izotova, T. G. Kinzy, and P. D. Nagy. 2011. Synergistic Roles of Eukaryotic Translation Elongation Factors 1Bgamma and 1A in Stimulation of Tombusvirus Minus-Strand Synthesis. PLoS Pathog 7:e1002438.

Pathak, K. B., J. Pogany, and P. D. Nagy. 2011. Non-template functions of the viral RNA in plant RNA virus replication. Curr Opin Virol 1:332-8

Nagy, P. D., R. Y. Wang, J. Pogany, A. Hafren, and K. Makinen. 2011. Emerging picture of host chaperone and cyclophilin roles in RNA virus replication. Virology 411:374-82.

Nagy, P. D. 2011. The roles of host factors in tombusvirus RNA recombination. Adv Virus Res 81:63-84.

Li, Z., and P. D. Nagy. 2011. Diverse roles of host RNA binding proteins in RNA virus replication. RNA Biol 8:305-15.

Jaag, H. M., Q. Lu, M. E. Schmitt, and P. D. Nagy. 2011. Role of RNase MRP in viral RNA degradation and RNA recombination. J Virol 85:243-53.

Huang, T. S., and P. D. Nagy. 2011. Direct inhibition of tombusvirus plus-strand RNA synthesis by a dominant negative mutant of a host metabolic enzyme, glyceraldehyde-3-phosphate dehydrogenase, in yeast and plants. J Virol 85:9090-102. 

2010

Xu, K., and P. D. Nagy. 2010. Dissecting Virus-Plant Interactions Through Proteomics Approaches. Current Proteomics 7:316-327.

Sharma, M., Z. Sasvari, and P. D. Nagy. 2010. Inhibition of sterol biosynthesis reduces tombusvirus replication in yeast and plants. J Virol 84:2270-81.

Sasvari, Z., and P. D. Nagy. 2010. Making of viral replication organelles by remodeling interior membranes. Viruses 2:2436-42.

Pogany, J., Panavas, T., Serviene, E., Nawaz-Ul-Rehman, MS., and Nagy, PD. 2010. A high-throughput approach for studying virus replication in yeast. Current Protocols in Microbiology 19:16J.1.1-16J.1.15.

Nagy, P. D., and J. Pogany. 2010. Global genomics and proteomics approaches to identify host factors as targets to induce resistance against Tomato bushy stunt virus. Adv Virus Res 76:123-77.

Mendu, V., M. Chiu, D. Barajas, Z. Li, and P. D. Nagy. 2010. Cpr1 cyclophilin and Ess1 parvulin prolyl isomerases interact with the tombusvirus replication protein and inhibit viral replication in yeast model host. Virology 406:342-51.

Li, Z., J. Pogany, S. Tupman, A. M. Esposito, T. G. Kinzy, and P. D. Nagy. 2010. Translation elongation factor 1A facilitates the assembly of the tombusvirus replicase and stimulates minus-strand synthesis. PLoS Pathog 6:e1001175.

Jiang, Y., Z. Li, and P. D. Nagy. 2010. Nucleolin/Nsr1p binds to the 3' noncoding region of the tombusvirus RNA and inhibits replication. Virology 396:10-20.

Jiang, Y., C. P. Cheng, E. Serviene, N. Shapka, and P. D. Nagy. 2010. Repair of lost 5' terminal sequences in tombusviruses: Rapid recovery of promoter- and enhancer-like sequences in recombinant RNAs. Virology 404:96-105.

Jaag, H. M., J. Pogany, and P. D. Nagy. 2010. A host Ca2+/Mn2+ ion pump is a factor in the emergence of viral RNA recombinants. Cell Host Microbe 7:74-81.

Jaag, H. M., and P. D. Nagy. 2010. The combined effect of environmental and host factors on the emergence of viral RNA recombinants. PLoS Pathog 6:e1001156.

Barajas, D., and P. D. Nagy. 2010. Ubiquitination of tombusvirus p33 replication protein plays a role in virus replication and binding to the host Vps23p ESCRT protein. Virology 397:358-68. 

2009

Wu, B., J. Pogany, H. Na, B. L. Nicholson, P. D. Nagy, and K. A. White. 2009. A discontinuous RNA platform mediates RNA virus replication: building an integrated model for RNA-based regulation of viral processes. PLoS Pathog 5:e1000323.

Wang, R. Y., J. Stork, J. Pogany, and P. D. Nagy. 2009. A temperature sensitive mutant of heat shock protein 70 reveals an essential role during the early steps of tombusvirus replication. Virology 394:28-38.

Wang, R. Y., J. Stork, and P. D. Nagy. 2009. A key role for heat shock protein 70 in the localization and insertion of tombusvirus replication proteins to intracellular membranes. J Virol 83:3276-87.

Sasvari, Z., S. Bach, M. Blondel, and P. D. Nagy. 2009. Inhibition of RNA recruitment and replication of an RNA virus by acridine derivatives with known anti-prion activities. PLoS One 4:e7376.

Pathak, K. B., and P. D. Nagy. 2009. Defective Interfering RNAs: Foes of Viruses and Friends of Virologists. Viruses 1:895-919.

Nagy, P. D. 2009. Yeast as a model host to explore plant virus - host interactions. Phytopathology99:S165-S165.

Li, Z., J. Pogany, T. Panavas, K. Xu, A. M. Esposito, T. G. Kinzy, and P. D. Nagy. 2009. Translation elongation factor 1A is a component of the tombusvirus replicase complex and affects the stability of the p33 replication co-factor. Virology 385:245-60.

Jaag, H. M., and P. D. Nagy. 2009. Silencing of Nicotiana benthamiana Xrn4p exoribonuclease promotes tombusvirus RNA accumulation and recombination. Virology 386:344-52.

Barajas, D., Z. Li, and P. D. Nagy. 2009. The Nedd4-type Rsp5p ubiquitin ligase inhibits tombusvirus replication by regulating degradation of the p92 replication protein and decreasing the activity of the tombusvirus replicase. J Virol 83:11751-64.

Barajas, D., Y. Jiang, and P. D. Nagy. 2009. A unique role for the host ESCRT proteins in replication of Tomato bushy stunt virus. PLoS Pathog 5:e1000705. 

2008

Wang, R. Y., and P. D. Nagy. 2008. Tomato bushy stunt virus co-opts the RNA-binding function of a host metabolic enzyme for viral genomic RNA synthesis. Cell Host Microbe 3:178-87.

Rajendran, K. S., and P. D. Nagy. 2008. Surface plasmon resonance analysis of interactions between replicase proteins of tomato bushy stunt virus. Methods Mol Biol 451:267-77.

Pogany, J., J. Stork, Z. Li, and P. D. Nagy. 2008. In vitro assembly of the Tomato bushy stunt virus replicase requires the host Heat shock protein 70. Proc Natl Acad Sci U S A 105:19956-61.

Pogany, J., and P. D. Nagy. 2008. Authentic replication and recombination of Tomato bushy stunt virus RNA in a cell-free extract from yeast. J Virol 82:5967-80.

Pathak, K. B., Z. Sasvari, and P. D. Nagy. 2008. The host Pex19p plays a role in peroxisomal localization of tombusvirus replication proteins. Virology 379:294-305.

Panavas, T., E. Serviene, J. Pogany, and P. D. Nagy. 2008. Genome-wide screens for identification of host factors in viral replication. Methods Mol Biol 451:615-24.

Nagy, P. D., and J. Pogany. 2008. Multiple roles of viral replication proteins in plant RNA virus replication. Methods Mol Biol 451:55-68.

Nagy, P. D. 2008. Yeast as a model host to explore plant virus-host interactions. Annu Rev Phytopathol46:217-42.

Li, Z., D. Barajas, T. Panavas, D. A. Herbst, and P. D. Nagy. 2008. Cdc34p ubiquitin-conjugating enzyme is a component of the tombusvirus replicase complex and ubiquitinates p33 replication protein. J Virol 82:6911-26.

2007

Jonczyk, M., K. B. Pathak, M. Sharma, and P. D. Nagy. 2007. Exploiting alternative subcellular location for replication: tombusvirus replication switches to the endoplasmic reticulum in the absence of peroxisomes. Virology 362:320-30.

Jaag, H. M., J. Stork, and P. D. Nagy. 2007. Host transcription factor Rpb11p affects tombusvirus replication and recombination via regulating the accumulation of viral replication proteins. Virology368:388-404.

Cheng, C. P., H. M. Jaag, M. Jonczyk, E. Serviene, and P. D. Nagy. 2007. Expression of the Arabidopsis Xrn4p 5'-3' exoribonuclease facilitates degradation of tombusvirus RNA and promotes rapid emergence of viral variants in plants. Virology 368:238-48.

2006

Serviene, E., Y. Jiang, C. P. Cheng, J. Baker, and P. D. Nagy. 2006. Screening of the yeast yTHC collection identifies essential host factors affecting tombusvirus RNA recombination. J Virol 80:1231-41.

Serva, S., and P. D. Nagy. 2006. Proteomics analysis of the tombusvirus replicase: Hsp70 molecular chaperone is associated with the replicase and enhances viral RNA replication. J Virol 80:2162-9.

Rajendran, K. S., and P. D. Nagy. 2006. Kinetics and functional studies on interaction between the replicase proteins of Tomato Bushy Stunt Virus: requirement of p33:p92 interaction for replicase assembly. Virology 345:270-9.

Panavas, T., J. Stork, and P. D. Nagy. 2006. Use of double-stranded RNA templates by the tombusvirus replicase in vitro: Implications for the mechanism of plus-strand initiation. Virology 352:110-20.

Nagy, P. D., and J. Pogany. 2006. Yeast as a model host to dissect functions of viral and host factors in tombusvirus replication. Virology 344:211-20.

Jiang, Y., E. Serviene, J. Gal, T. Panavas, and P. D. Nagy. 2006. Identification of essential host factors affecting tombusvirus RNA replication based on the yeast Tet promoters Hughes Collection. J Virol80:7394-404.

Cheng, C. P., E. Serviene, and P. D. Nagy. 2006. Suppression of viral RNA recombination by a host exoribonuclease. J Virol 80:2631-40.

2005

Stork, J., Z. Panaviene, and P. D. Nagy. 2005. Inhibition of in vitro RNA binding and replicase activity by phosphorylation of the p33 replication protein of Cucumber necrosis tombusvirus. Virology 343:79-92.

Shapka, N., J. Stork, and P. D. Nagy. 2005. Phosphorylation of the p33 replication protein of Cucumber necrosis tombusvirus adjacent to the RNA binding site affects viral RNA replication. Virology 343:65-78.

Serviene, E., N. Shapka, C. P. Cheng, T. Panavas, B. Phuangrat, J. Baker, and P. D. Nagy. 2005. Genome-wide screen identifies host genes affecting viral RNA recombination. Proc Natl Acad Sci U S A102:10545-50.

Pogany, J., K. A. White, and P. D. Nagy. 2005. Specific binding of tombusvirus replication protein p33 to an internal replication element in the viral RNA is essential for replication. J Virol 79:4859-69.

Panaviene, Z., T. Panavas, and P. D. Nagy. 2005. Role of an internal and two 3'-terminal RNA elements in assembly of tombusvirus replicase. J Virol 79:10608-18.

Panavas, T., E. Serviene, J. Brasher, and P. D. Nagy. 2005. Yeast genome-wide screen reveals dissimilar sets of host genes affecting replication of RNA viruses. Proc Natl Acad Sci U S A 102:7326-31.

Panavas, T., and P. D. Nagy. 2005. Mechanism of stimulation of plus-strand synthesis by an RNA replication enhancer in a tombusvirus. J Virol 79:9777-85.

Panavas, T., C. M. Hawkins, Z. Panaviene, and P. D. Nagy. 2005. The role of the p33:p33/p92 interaction domain in RNA replication and intracellular localization of p33 and p92 proteins of Cucumber necrosis tombusvirus. Virology 338:81-95.

Monkewich, S., H. X. Lin, M. R. Fabian, W. Xu, H. Na, D. Ray, O. A. Chernysheva, P. D. Nagy, and K. A. White. 2005. The p92 polymerase coding region contains an internal RNA element required at an early step in Tombusvirus genome replication. J Virol 79:4848-58.

Cheng, C. P., T. Panavas, G. Luo, and P. D. Nagy. 2005. Heterologous RNA replication enhancer stimulates in vitro RNA synthesis and template-switching by the carmovirus, but not by the tombusvirus, RNA-dependent RNA polymerase: implication for modular evolution of RNA viruses. Virology 341:107-21.

2004

White, K. A., and P. D. Nagy. 2004. Advances in the molecular biology of tombusviruses: gene expression, genome replication, and recombination. Prog Nucleic Acid Res Mol Biol 78:187-226.

Shapka, N., and P. D. Nagy. 2004. The AU-rich RNA recombination hot spot sequence of Brome mosaic virus is functional in tombusviruses: implications for the mechanism of RNA recombination. J Virol78:2288-300.

Rajendran, K. S., and P. D. Nagy. 2004. Interaction between the replicase proteins of Tomato bushy stunt virus in vitro and in vivo. Virology 326:250-61.

Panaviene, Z., T. Panavas, S. Serva, and P. D. Nagy. 2004. Purification of the cucumber necrosis virus replicase from yeast cells: role of coexpressed viral RNA in stimulation of replicase activity. J Virol78:8254-63.

Boonrod, K., D. Galetzka, P. D. Nagy, U. Conrad, and G. Krczal. 2004. Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance. Nat Biotechnol 22:856-62.

Rajendran, K. S., and P. D. Nagy. 2003. Characterization of the RNA-binding domains in the replicase proteins of tomato bushy stunt virus. J Virol 77:9244-58.

Pogany, J., M. R. Fabian, K. A. White, and P. D. Nagy. 2003. A replication silencer element in a plus-strand RNA virus. EMBO J 22:5602-11.

Panaviene, Z., and P. D. Nagy. 2003. Mutations in the RNA-binding domains of tombusvirus replicase proteins affect RNA recombination in vivo. Virology 317:359-72.

2003

Panaviene, Z., J. M. Baker, and P. D. Nagy. 2003. The overlapping RNA-binding domains of p33 and p92 replicase proteins are essential for tombusvirus replication. Virology 308:191-205.

Panavas, T., Z. Panaviene, J. Pogany, and P. D. Nagy. 2003. Enhancement of RNA synthesis by promoter duplication in tombusviruses. Virology 310:118-29.

Panavas, T., and P. D. Nagy. 2003. Yeast as a model host to study replication and recombination of defective interfering RNA of Tomato bushy stunt virus. Virology 314:315-25.

Panavas, T., and P. D. Nagy. 2003. The RNA replication enhancer element of tombusviruses contains two interchangeable hairpins that are functional during plus-strand synthesis. J Virol 77:258-69.

Cheng, C. P., and P. D. Nagy. 2003. Mechanism of RNA recombination in carmo- and tombusviruses: evidence for template switching by the RNA-dependent RNA polymerase in vitro. J Virol 77:12033-47.

2002

Rajendran, K. S., J. Pogany, and P. D. Nagy. 2002. Comparison of turnip crinkle virus RNA-dependent RNA polymerase preparations expressed in Escherichia coli or derived from infected plants. J Virol76:1707-17.

Panavas, T., J. Pogany, and P. D. Nagy. 2002. Internal initiation by the cucumber necrosis virus RNA-dependent RNA polymerase is facilitated by promoter-like sequences. Virology 296:275-87.

Panavas, T., J. Pogany, and P. D. Nagy. 2002. Analysis of minimal promoter sequences for plus-strand synthesis by the Cucumber necrosis virus RNA-dependent RNA polymerase. Virology 296:263-74.

Cheng, C. P., J. Pogany, and P. D. Nagy. 2002. Mechanism of DI RNA formation in tombusviruses: dissecting the requirement for primer extension by the tombusvirus RNA dependent RNA polymerase in vitro. Virology 304:460-73.

2001

Nagy, P. D., J. Pogany, and A. E. Simon. 2001. In vivo and in vitro characterization of an RNA replication enhancer in a satellite RNA associated with turnip crinkle virus. Virology 288:315-24.

2000

Yoshinari, S., P. D. Nagy, A. E. Simon, and T. W. Dreher. 2000. CCA initiation boxes without unique promoter elements support in vitro transcription by three viral RNA-dependent RNA polymerases. RNA6:698-707.

Nagy, P. D., and J. Pogany. 2000. Partial purification and characterization of Cucumber necrosis virus and Tomato bushy stunt virus RNA-dependent RNA polymerases: similarities and differences in template usage between tombusvirus and carmovirus RNA-dependent RNA polymerases. Virology 276:279-88.

1999

Nagy, P. D., J. Pogany, and A. E. Simon. 1999. RNA elements required for RNA recombination function as replication enhancers in vitro and in vivo in a plus-strand RNA virus. EMBO J 18:5653-65.

Nagy, P. D., C. Ogiela, and J. J. Bujarski. 1999. Mapping sequences active in homologous RNA recombination in brome mosaic virus: prediction of recombination hot spots. Virology 254:92-104.

1998

Nagy, P. D., C. Zhang, and A. E. Simon. 1998. Dissecting RNA recombination in vitro: role of RNA sequences and the viral replicase. EMBO J 17:2392-403.

Nagy, P. D., and A. E. Simon. 1998. In vitro characterization of late steps of RNA recombination in turnip crinkle virus.II. The role of the priming stem and flanking sequences. Virology 249:393-405.

Nagy, P. D., and A. E. Simon. 1998. In vitro characterization of late steps of RNA recombination in turnip crinkle virus. I. Role of motif1-hairpin structure. Virology 249:379-92.

Nagy, P. D., and J. J. Bujarski. 1998. Silencing homologous RNA recombination hot spots with GC-rich sequences in brome mosaic virus. J Virol 72:1122-30.

Figlerowicz, M., P. D. Nagy, N. Tang, C. C. Kao, and J. J. Bujarski. 1998. Mutations in the N terminus of the brome mosaic virus polymerase affect genetic RNA-RNA recombination. J Virol 72:9192-200.

1997

Nagy, P. D., and A. E. Simon. 1997. New insights into the mechanisms of RNA recombination. Virology235:1-9.

Nagy, P. D., C. D. Carpenter, and A. E. Simon. 1997. A novel 3'-end repair mechanism in an RNA virus. Proc Natl Acad Sci U S A 94:1113-8.

Nagy, P. D., and J. J. Bujarski. 1997. Engineering of homologous recombination hotspots with AU-rich sequences in brome mosaic virus. J Virol 71:3799-810.

Figlerowicz, M., P. D. Nagy, and J. J. Bujarski. 1997. A mutation in the putative RNA polymerase gene inhibits nonhomologous, but not homologous, genetic recombination in an RNA virus. Proc Natl Acad Sci U S A 94:2073-8.

1996

Simon, A. E., and P. D. Nagy. 1996. RNA recombination in turnip crinkle virus: Its sole in formation of chimeric RNAs, multimers, and in 3'-end repair. Seminars in Virology 7:373-379.

Nagy, P. D., and J. J. Bujarski. 1996. Homologous RNA recombination in brome mosaic virus: AU-rich sequences decrease the accuracy of crossovers. J Virol 70:415-26.

Bujarski, J. J., and P. D. Nagy. 1996. Different mechanisms of homologous and nonhomologous recombination in brome mosaic virus: Role of RNA sequences and replicase proteins. Seminars in Virology 7:363-372.

1995

Nagy, P. D., A. Dzianott, P. Ahlquist, and J. J. Bujarski. 1995. Mutations in the helicase-like domain of protein 1a alter the sites of RNA-RNA recombination in brome mosaic virus. J Virol 69:2547-56.

Nagy, P. D., and J. J. Bujarski. 1995. Efficient system of homologous RNA recombination in brome mosaic virus: sequence and structure requirements and accuracy of crossovers. J Virol 69:131-40.

1994

Pogany, J., Q. Huang, J. Romero, P. D. Nagy, and J. J. Bujarski. 1994. Infectious transcripts from PCR-amplified broad bean mottle bromovirus cDNA clones and variable nature of leader regions in RNA 3 segment. J Gen Virol 75 ( Pt 3):693-7.

Bujarski, J. J., P. D. Nagy, and S. Flasinski. 1994. Molecular studies of genetic RNA-RNA recombination in brome mosaic virus. Adv Virus Res 43:275-302.

Bujarski, J. J., and P. D. Nagy. 1994. Targeting of the site of nonhomologous genetic recombination in brome mosaic virus. Arch Virol Suppl 9:231-8.

1993

Nagy, P. D., and J. J. Bujarski. 1993. Targeting the site of RNA-RNA recombination in brome mosaic virus with antisense sequences. Proc Natl Acad Sci U S A 90:6390-4.

1992

Nagy, P. D., and J. J. Bujarski. 1992. Genetic recombination in brome mosaic virus: effect of sequence and replication of RNA on accumulation of recombinants. J Virol 66:6824-8.

1991

Nagy, P. D., and R. Gaborjanyi. 1991. Evidence for Cross-Protection between Strains of Barley Stripe Mosaic-Virus. Acta Phytopathologica Et Entomologica Hungarica 26:193-197.

Nagy, P. D., and R. Gaborjanyi. 1991. Characterization of Barley Stripe Mosaic-Virus Hungarian Strain. Acta Phytopathologica Et Entomologica Hungarica 26:187-192.

Milinko, I., R. Gaborjanyi, and P. D. Nagy. 1991. Proceedings of the 5th Conference on Virus Diseases of Gramineae in Europe Held in Budapest, Hungary 24-27 May, 1988 - Preface. Acta Phytopathologica Et Entomologica Hungarica 26:7-7.

Gaborjanyi, R., J. Szirmai, L. Beczner, and P. D. Nagy. 1991. Virus Diseases of Gramineae in Hungary. Acta Phytopathologica Et Entomologica Hungarica 26:83-86.

1990

Nagy, P. D., and R. Gaborjanyi. 1990. Characterization of 2 Unusual Strains of Barley Stripe Mosaic-Virus. Novenytermeles 39:235-243.

Burgyan, J., P. D. Nagy, and M. Russo. 1990. Synthesis of infectious RNA from full-length cloned cDNA to RNA of cymbidium ringspot tombusvirus. J Gen Virol 71 ( Pt 8):1857-60.

1989

Nagy, P. D., R. Gaborjanyi, L. Kovacs, and I. Farkas. 1989. Antiviral activity of tiazofurine against barley stripe mosaic virus. Antiviral Res 11:41-5.

Adam, A., and P. D. Nagy. 1989. Variations in Membrane Polar Lipids of Barley Leaves Infected with 3 Strains of Barley Stripe Mosaic-Virus and with Poa Semilatent Virus. Plant Science 61:53-59.

1988

Gaborjanyi, R., and P. D. Nagy. 1988. Wheat Streak Mosaic-Virus in Hungary. Novenytermeles 37:391-395.

Nagy, P., and J. Milinko. 1986. Some New Data on the Epidemiology of Barley Yellow Dwarf Virus in Hungary. Novenytermeles 35:493-500.